Okwute Simon K., Okolo Simon C., Okoh-Esene R., Olajide Olutayo O.
Department of Chemistry, University of Abuja, P.M. B. 117, Gwagwalada, Abuja, F.C.T, Nigeria; Chemistry Advanced Laboratory, Sheda Science and Technology Complex, Abuja, F.C.T, Nigeria
Okwute Simon, K., Department of Chemistry, University of Abuja, P.M. B. 117, Gwagwalada, Abuja, F.C.T, Nigeria; Okolo Simon, C., Chemistry Advanced Laboratory, Sheda Science and Technology Complex, Abuja, F.C.T, Nigeria; Okoh-Esene, R., Chemistry Advanced Laboratory, Sheda Science and Technology Complex, Abuja, F.C.T, Nigeria; Olajide Olutayo, O., Chemistry Advanced Laboratory, Sheda Science and Technology Complex, Abuja, F.C.T, Nigeria
The leaf of Phyllanthus amarus was investigated for its chemical and biological properties. The powdered leaf was extracted with 95% ethanol and the crude extract was partitioned successively with hexane, dichloromethane, ethyl acetate and 70% aqueous methanol. Phytochemical analysis of the crude extract showed that it contained alkaloids, balsams, flavonoids, saponins, steroids, tannins, terpenoids, and anthraquinones. The proximate analysis revealed that Phyllanthus amarus leaf is a good source of carbohydrates, proteins and fibre. The crude extract and fractions were tested against Pseudomonas aeruginosa,Escherichia coli, Staphylococcous aureus, and Candida albicans for their antimicrobial and antifungal activities. Flash column chromatographic separation of the antimicrobial dichloromethane fraction gave a partially pure isolate which on GC-MS analysis revealed the presence of 2- naphthyl-α- D-galactopyranoside and 9-octadecenoic acid in the leaf extract. © 2014-2015, Sphinx Knowledge House. All rights reserved.
alkaloid; anthraquinone; balsam; carbohydrate; flavonoid; Phyllanthus amarus extract; protein; saponin; steroid; tannin; terpenoid; antimicrobial activity; Article; bioassay; Candida albicans; chemical analysis; chemical composition; chromatography; Escherichia coli; mass fragmentography; nonhuman; phytochemistry; Pseudomonas aeruginosa; quantitative analysis; Staphylococcus aureus