Sobti R.C., Berhane N., Melese S., Mahdi S.A., Gupta L., Thakur H., Singh N.
Department of Biotechnology, Panjab University, 160014 Chandigarh, India; Department of Biotechnology, University of Gondar, Gondar, Ethiopia; Department of Mathematics, University of Gondar, Gondar, Ethiopia
Sobti, R.C., Department of Biotechnology, Panjab University, 160014 Chandigarh, India; Berhane, N., Department of Biotechnology, University of Gondar, Gondar, Ethiopia; Melese, S., Department of Mathematics, University of Gondar, Gondar, Ethiopia; Mahdi, S.A., Department of Biotechnology, Panjab University, 160014 Chandigarh, India; Gupta, L., Department of Biotechnology, Panjab University, 160014 Chandigarh, India; Thakur, H., Department of Biotechnology, Panjab University, 160014 Chandigarh, India; Singh, N., Department of Biotechnology, Panjab University, 160014 Chandigarh, India
Prostate cancer is the second most diagnosed cancer in men next to skin cancer in the developed world. Risk of disease varies most prominently with age, ethnicity, family history, and diet. Genetic polymorphism of some genes has been implicated in increasing the risk. The XPD (Xeroderma pigmentosum group D) gene codes for a DNA helicase involved in transcription and nucleotide excision repair. The aim of this study is to evaluate the effect of XPD 751 Lys/Gln polymorphism on risk of prostate cancer on north Indian patients. Blood sample from 150 prostate cancer patients, 150 from Prostate Hyper Plasia and equal number of samples from healthy control groups was collected from North India. The polymerase chain reaction and restrictive fragment length polymorphism techniques were implemented. Statistically nonsignificant increase risk of prostate cancer was observed with patients having Gln/Gln genotype (OR 1.62, 95% CI). © 2011 Springer Science+Business Media, LLC.
genomic DNA; xeroderma pigmentosum group C protein; glutamine; lysine; xeroderma pigmentosum group D protein; adult; aged; article; blood sampling; cancer genetics; cancer patient; cancer risk; controlled study; exon; gene frequency; gene locus; genetic association; genotype; high risk population; human; India; major clinical study; male; occupational hazard; peripheral lymphocyte; polymerase chain reaction; population genetics; prostate cancer; prostate hypertrophy; restriction fragment length polymorphism; sedentary lifestyle; single nucleotide polymorphism; clinical evaluation; confidence interval; DNA polymorphism; human cell; human tissue; prostate cancer; risk; statistical significance; Aged; Aged, 80 and over; Base Sequence; DNA Repair; DNA-Binding Proteins; Gene Frequency; Genetic Predisposition to Disease; Genotype; Humans; India; Male; Middle Aged; Polymorphism, Single Nucleotide; Prostatic Neoplasms; Risk; Risk Factors; Sequence Analysis, DNA; Xeroderma Pigmentosum Group D Protein