Department of Biochemistry, University of Zimbabwe, Biomolecular Interactions Analyses Group, P.O. Box MP 167, Mt. Pleasant, Harare, Zimbabwe
Chimponda, T., Department of Biochemistry, University of Zimbabwe, Biomolecular Interactions Analyses Group, P.O. Box MP 167, Mt. Pleasant, Harare, Zimbabwe; Mukanganyama, S., Department of Biochemistry, University of Zimbabwe, Biomolecular Interactions Analyses Group, P.O. Box MP 167, Mt. Pleasant, Harare, Zimbabwe
Increased expression of hematopoietic prostaglandin D2 synthase (H-PGDS) is responsible for allergic reactions promoting the inflammatory processes. A total of fifteen plant extracts were screened for their inhibitory activity against H-PGDS using 1-chloro-2, 4-dinitrobenzene (CDNB) as a substrate at 0.3 mg.mL-1. Seven of these plant extracts that were effective inhibitors of H-PGDS were then used to determine the half maximum inhibitory concentrations (IC<inf>50</inf>s). Parinari curatellifolia leaf ethanol extract was the most potent plant extract with an IC<inf>50</inf> of 3.8μg.mL-1 that was comparable to 2.1 μg.mL-1 of the standard Cibacron blue. P. curatellifolia extract directly inhibited H-PGDS activity, which was a kind of reversible inhibition of mixed type with respect to both GSH and CDNB as substrates. The results obtained in this study validate the ethnomedicinal use of P. curatellifolia in the reduction of symptoms of inflammation. Copyright © 2015 Taylor & Francis Group, LLC.
alcohol; cibacron blue f3ga; methanol; Parinari curatellifolia extract; plant extract; prostaglandin D synthase; prostaglandin D2; prostaglandin synthase inhibitor; unclassified drug; water; allergy; enzyme activity; gene expression; hematology; plant extract; antiinflammatory activity; Article; drug screening; enzyme inhibition; immobilized metal affinity chromatography; solvent extraction; Zimbabwe; Parinari