Ebigwai J.K., Ilondu E.M., Markson A., Ekeleme E.
In vitro evaluation of the essential oil extract of six plant species and ivermectin on the microfilaria larva of Simulium yahense
Research Journal of Medicinal Plant
Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
Ebigwai, J.K., Department of Biochemistry, University of Nigeria, Nsukka, Nigeria; Ilondu, E.M., Department of Biochemistry, University of Nigeria, Nsukka, Nigeria; Markson, A., Department of Biochemistry, University of Nigeria, Nsukka, Nigeria; Ekeleme, E., Department of Biochemistry, University of Nigeria, Nsukka, Nigeria
A comparative laboratory bio as say of the essential oil extracts of M. koenigii (seed), Citrus paradisi (seed) and the leaves, stem barks and roots of Alstonia boonei, Alternanthera repens, Eclipta prostrata and Rothmannia longiflora and their combination were compared with ivermectin against the third infectious microfilaria larva of Simulium yahense. Bioassay test showed remarkable larvicidal properties for ivermectin and the plant species under study as they could induce significant mortalities at low and varying concentrations in the larva of S. yahense. The LC50 and LC90 values estimated for M. koenigii, Citrus paradisi, Alstonia boonei, Alternanthera repens, Eclipta prostrata, Rothmannia longiflora, their combination and ivermectin are 5.75 and 13.18, 8.38 and 15.49, 3.02 and 15.49, 2.472 and 9.33, 2.87 and 12.88, 2.93 and 13.49, 2.61 and 3.16 and 2.43 and 5.37 ppm, respectively. The study revealed that ivermectin and the combination of the plant extracts could induce 100% mortality of the larva at a dose dependent response. © 2012 Academic Journals Inc.
Combination; Essential oil extracts; Ivermectin; Onchocerciasis; Simulium yahense
Alstonia boonei essential oil; Alternanthera repens essential oil; Citrus paradisi essential oil; Eclipta prostata essential oil; essential oil; ivermectin; larvicidal agent; Murraya koenigii essential oil; Rothmannia longiflora essential oil; unclassified drug; Alstonia; alstonia boonei; Alternanthera repens; article; bark; concentration response; controlled study; drug concentration; Eclipta; Eclipta prostata; grapefruit; in vitro study; LC 50; LC 90; medicinal plant; microfilaria (nematode larva); Murraya koenigii; nonhuman; plant leaf; plant root; plant seed; plant stem; Rothmannia longiflora; Simulium; simulium yahense; Alstonia boonei; Alternanthera pungens; Citrus x paradisi; Eclipta prostrata; Rothmannia longiflora; Simulium yahense