Evaluation of four Cameroonian medicinal plants for anticancer, antigonorrheal and antireverse transcriptase activities
Environmental Toxicology and Pharmacology
Department of Biochemistry, Faculty of Science, University of Yaoundé I, Cameroon; Department of Biochemistry, Faculty of Science, University of Dschang, Cameroon; Department of Organic Chemistry, Faculty of science, University of Yaoundé I, Cameroon; Department of Plant Science, Faculty of Agricultural and Biological Science, Pretoria 0002, South Africa
Methanol extracts from the leaves, bark and roots of four Cameroonian medicinal plants, Bersama engleriana, Cupressus lusitanica, Vitellaria paradoxa and Guibourtia tessmannii were tested for their in vitro cytotoxicity, antigonorrheal and antireverse transcriptase activities. The XTT (2,3-bis[2-methoxy-4-nitro-5-sulfophenyl]-2H-tetrazolium-5-carboxyanilide inner salt) assay, the dilution method and reverse transcriptase (RT) assay were used for the investigations. Preliminary phytochemical analysis of the extracts was also conducted using standard methods. Results showed that all extracts contained compounds belonging to the classes of phenols and terpenoids. They were also able to reduce in dose dependent manner, the proliferation of the cancer THP-1, DU145, HeLa, MCF-7, HepG2 and the normal Vero cells. IC50 values below 30μg/ml were noted with extract from the three parts of B. engleriana on at least two of the five studied cancer cell lines, the lowest value of 5.9μg/ml being obtained with sample from the bark. IC50 values below 30μg/ml were also recorded with extracts from the leaves (on HeLa cells) and bark (on MCF-7) of G. tessmanii, and that from the bark of C. lusitanica on MCF-7. Extracts from B. engleriana and those from the bark of V. paradoxa gave the minimal inhibitory concentrations (MIC) values below 100μg/ml on most of the 10 tested Nesseria gonorrhoeae strains. Extracts from B. engleriana also inhibited more than 80% the activity of the Human Immuno-deficiency Virus (HIV) enzyme. Finally, the results of the present study provide baseline information for the use of B. engleriana, C. lusitanica, G. tessmanii, V. paradoxa. © 2011 Elsevier B.V.
2,3 bis[2 methoxy 4 nitro 5 sulfophenyl] 2h tetrazolium 5 carboxyanilide inner salt; anilide; anthraquinone derivative; antineoplastic agent; Bersama engleriana extract; Cupressus lusitanica extract; doxorubicin; flavonoid; Guibourtia tessmannii extract; methanol; phenol derivative; plant extract; RNA directed DNA polymerase inhibitor; tannin derivative; terpenoid derivative; unclassified drug; Vitellaria paradoxa extract; animal cell; antibacterial activity; antineoplastic activity; article; bacterial strain; bark; Bersama engleriana; Cameroon; cancer cell culture; cancer inhibition; cell strain DU145; cell strain HepG2; cell strain MCF 7; cell strain THP 1; concentration response; controlled study; Cupressus; Cupressus lusitanica; dilution; drug cytotoxicity; drug isolation; drug screening; enzyme assay; enzyme inhibition; Guibourtia tessmannii; HeLa cell; human; human cell; IC 50; in vitro study; medicinal plant; minimum inhibitory concentration; Neisseria gonorrhoeae; nonhuman; phytochemistry; plant leaf; plant root; priority journal; Vero cell; Vitellaria paradoxa; Animals; Anti-Bacterial Agents; Antineoplastic Agents; Cameroon; Cell Line, Tumor; Cercopithecus aethiops; Gonorrhea; HIV Reverse Transcriptase; Humans; Microbial Sensitivity Tests; Neisseria gonorrhoeae; Neoplasms; Phenols; Plant Extracts; Plants, Medicinal; Reverse Transcriptase Inhibitors; Terpenes; Vero Cells; Bersama; Cupressus lusitanica; Guibourtia tessmannii; Neisseria gonorrhoeae; Vitellaria paradoxa