Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 570, South Africa; Department of Microbiology and Parasitology, Faculty of Science, University of Buea, P.O. Box 63, Buea, Cameroon
Okeleye, B.I., Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 570, South Africa; Mkwetshana, N.T., Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 570, South Africa; Ndip, R.N., Department of Biochemistry and Microbiology, Faculty of Science and Agriculture, University of Fort Hare, Private Bag X1314, Alice 570, South Africa, Department of Microbiology and Parasitology, Faculty of Science, University of Buea, P.O. Box 63, Buea, Cameroon
We assessed the in vitro antimicrobial activity of Peltophorum africanum by means of the agar well and macrodilution methods. The toxicity on a normal human liver cell (Chang liver cell) was determined using the CellTiter-Blue cell viability assay, and the compounds contained in the fractions were identified using GC-MS. Zone diameter of inhibition of the extract ranged from 12.5 ± 0.7 to 32 ± 2.8 mm for bacteria and from 7.5 ± 0.7 to 26.4 ± 3.4 mm for yeast. Marked activity of the extract was observed against Plesiomonas shigelloides ATCC 51903, with MIC and MLC values of 0.15625 and 0.3125 mg/mL, respectively. The extract was both bactericidal (MIC index ≤ 2) and bacteriostatic/fungistatic (MIC index > 2) in activity. Lethal dose at 50 (LD50) showed 82.64 ± 1.40 degree of toxicity at 24 hrs, and 95 percentile of cell death dose activity ranged from log 3.12 ± 0.01 to 4.59 ± 0.03. The activity of the eight fractions tested ranged from 1.0 ± 0.5 to 3.7 ± 1.6 mg/mL (IC50) and from 2.1 ± 0.8 to 6.25 ± 0 mg/mL (IC90). The extract was toxic to human Chang liver cell lines. © 2013 Benjamin I. Okeleye et al.
Peltophorum africanum extract; plant extract; unclassified drug; antifungal agent; antiinfective agent; plant extract; agar dilution; antibacterial activity; antifungal activity; article; bactericidal activity; cell death; controlled study; drug cytotoxicity; human; human cell; LD 50; liver cell culture; mass fragmentography; medicinal plant; minimum inhibitory concentration; nonhuman; Peltophorum africanum; Plesiomonas shigelloides; bacterial phenomena and functions; Caesalpinia; cell survival; chemistry; drug effect; fungus; liver tumor; pathology; physiology; treatment outcome; tumor cell line; Peltophorum africanum; Plesiomonas shigelloides; Anti-Bacterial Agents; Antifungal Agents; Bacterial Physiological Phenomena; Caesalpinia; Cell Line, Tumor; Cell Survival; Fungi; Humans; Liver Neoplasms; Plant Extracts; Treatment Outcome