Evaluation of the antibacterial activity of extracts of Sida acuta against clinical isolates of Staphylococcus aureus isolated from Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome patients
Research Journal of Pharmacology
Department of Applied Microbiology, Ebonyi State University, PMB 053, Abakaliki, Nigeria
Abstract: Antimicrobial activity of aqueous and ethanol leaves extracts of Sida acuta against 45 clinical isolates of Staphylococccus aureas isolated from nasal cavity of Human Immunodeficiency Virus/Acquired Immunodeficiency Syndrome. (HIV/AIDS) patients from University of Nigeria teaching hospital, Enugu was evaluated using agar well diffusion method. The Minimum Inhibitory Concentration (MIC) of the extracts was also determined using agar well diffusion technique and the killing rates of each extracts was also determined at different time intervals of 0-90 min. Results of the agar well diffusion study revealed that ethanol extracts produced the highest antimicrobial activity (86%), followed by hot water (61 %) and cold water extracts (48%). The MIC obtained ranged from 0.9625-1.8125 μg mL-1 for ethanol extracts, 7.8125-31.25 μg mL-1 for hot water and 15.625-31.25 μg mL-1 for cold water extracts. The result of killing rate studies showed that the test organisms were killed within 0-10 min for ethanol and hot water extracts and 5-60 min for cold water extracts. The over all results indicated that Sida acuta extracts have appreciable antimicrobial activity against S. aureus isolated Eroin HIV/AIDS patients. In addition to authenticating, the folkloric use of Sida acuta in the treatment of common diseases, the finding of this study highlights the possible usefulness of this plant material in the treatment of opportunistic infections caused by S. aureus in HIV/AIDS patients. © Medwell Journals, 2009.
alcohol; cold water; herbaceous agent; hot water; lincomycin; Sida acuta extract; unclassified drug; water; agar diffusion; angiosperm; antibacterial activity; article; bacterial kinetics; bacterial strain; bacterium isolate; controlled study; human; Human immunodeficiency virus infected patient; inhibition kinetics; minimum inhibitory concentration; nonhuman; nose cavity; Sida acuta; Staphylococcus aureus