Stereological evaluation of the effects of momordica charantia, antioxidants and testosterone on seminiferous tubules of rat [Evaluación estereológica de los efectos de momordica charantia, antioxidantes y testosterona sobre los túbulos seminíferos de rat
International Journal of Morphology
Department of Anatomy, Faculty of Basic Medical Sciences, University of Lagos, Idi-Araba, Lagos, Nigeria
Ubiquitous consumption of Momordica charantia is hinged on credence of its potency in alleviating ailments. Almost no data exist on its quantitative relationship to the testes. The aim was to study the histomorphometric effect of Momordica charantia seed extract (MC), ascorbic acid (C), alpha-tocopherol (E) and testosterone (T) on the testes of rats. Ninety five mature 6-8 week-old male Sprague-Dawley (S-D) rats allotted randomly into six groups. Group I: fed MC for 16 weeks. Group II: administered MC for 8 weeks, later distilled water for another 8 weeks. Group III: administered C, E, T and a combination of all three test solutions (CET) for 8 weeks and later fed MC for another 8 weeks. Group IV: received MC initially for 8 weeks and later administered C, E, T and CET for another 8 weeks. Group V: fed concurrently the extract, C, E, T and CET for 8 weeks. Group VI (control): administered C, E, T, CET and distilled water for 16 weeks. The doses for MC, C, E and T were 50 mg/100 g b.w/oral/day, 0.01, 20.0 and 0.05 mg/kg. The animals were sacrificed; testes estimated for volume. Diameter, cross sectional area (AC), number of profiles per unit area (NA), length density (LV) and numerical density (NV) of the seminiferous tubules determined. The mean testicular volumes, tubular diameters and AC showed significant decline (p ≤ 0.05) in Groups I and III compared to controls. On cessation of MC values recovered to baseline control value. Groups IV and V, were not significantly (p £ 0.05) different from controls. There was a significant (p ≤ 0.05) increase in NA, LV and NV of seminiferous tubules in Groups I and III compared to controls. Histomorphometric data supports a reversible deleterious effect of MC on testes.