Impact of alcohol on male reproductive hormones, oxidative stress and semen parameters in Sprague-Dawley rats
Middle East Fertility Society Journal
Department of Anatomy, College of Medicine, University of Lagos, Lagos State, Nigeria; Department of Anatomy, College of Health Sciences, Benue State University, Makurdi, Benue State, Nigeria
Objective To investigate the impact of alcohol on the reproductive hormones, oxidative stress and semen parameters. Design This is an experimental animal study. Materials and methods Adult male Sprague-Dawley rats weighing between 170 and 200 g received 30% v/v ethanol at a concentration of 2 g/kg body weight for a period of 4 weeks, 8 weeks and 16 weeks. Parameters tested include: testosterone, estrogen, luteinizing hormone (LH), follicle stimulating hormone (FSH), gonadotropin hormone releasing hormone (GnRH), malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH), sperm count and sperm motility. Results After the 4 week study, there was a significant decrease (p < 0.05) in estrogen levels, sperm count and sperm motility. Testosterone levels also decreased while MDA levels increased significantly. After the 8 week study, testosterone levels decreased significantly, LH and FSH also decreased but GnRH levels increased significantly. MDA and SOD levels increased significantly but sperm count and sperm motility decreased significantly compared to controls. After the 16 week study, testosterone and GnRH levels decreased significantly compared to controls. MDA levels increased significantly while sperm count and motility decreased significantly. Conclusion Acute and chronic administration of alcohol depletes testosterone levels, increases oxidative stress and decreases semen parameters. This impact of alcohol on testosterone levels is mediated by direct testicular toxicity and by altering the hormone feedback system in the pituitary gland and the hypothalamus.
alcohol; estrogen; follitropin; glutathione; gonadorelin; luteinizing hormone; malonaldehyde; superoxide dismutase; testosterone; adult; alcohol consumption; animal cell; animal experiment; Article; concentration (parameters); controlled study; feedback system; hormone action; hormone determination; hypophysis; male; male infertility; nonhuman; oxidative stress; protein analysis; rat; spermatozoon count; spermatozoon motility; testis disease; tissue level