Overview of parameters influencing biomass and bioreactor performance used for extracellular ligninase production from Phanerochaete chrysosporium
Brazilian Archives of Biology and Technology
Department of Agriculture and Food Sciences, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 8000, South Africa; Department of Chemical Engineering, Cape Peninsula University of Technology, P.O. Box 652, Cape Town, 8000, South Africa; Department of Microbiology, University of Stellenbosch, P/Bag X1, Matieland, 7602, South Africa
The production of extracellular enzymes is gaining momentum as commercial interests seek alternative ways to improve the productivity in the biotechnology and pharmaceutical industries. Early research studies looked at improving batch bioreactor operational challenges; however, the use of continuous cultures was indicated to be favourable. This led to a new approach developed to produce extracellular enzymes continuously using fixed-film bioreactors from biofilms immobilised on polymeric and inorganic membranes. In this review, the performance of P. chrysosporium biomass, evaluated in terms of ligninase production using different bioreactor operation conditions, is highlighted. Furthermore, the limitations related to the implementation of optimised batch culture conditions to continuous fixed-film bioreactors are discussed. DO transportation, trace element toxicity and lipid peroxidation effects on P. chrysosporium biomass in fixed-film bioreactors operated for elongated periods, are also discussed.
Bacteria (microorganisms); Chrysosporium; Phanerochaete; Phanerochaete chrysosporium